ANSIG version 3.3


Assignment of NMR Spectra
by Interactive Graphics

A program written in Fortran 77 for Silicon Graphics machines.

Copyright © 1996 Per Kraulis


How to obtain ANSIG

Command reference

Caveat: This documentation is not perfect. Some parts are not finished, some are not very well written. If you find any specific problems, please email them to Per Kraulis so that the problems can be fixed in the next release.

References

A paper describing the 3D/4D version of the ANSIG program as such is planned, but has not yet been written. The JMR paper is the original ANSIG paper, while the Biochemistry paper uses the 3D/4D version and describes some features of ANSIG in the methods part.

In publications, refer to:

P.J. Kraulis, ANSIG: A Program for the Assignment of Protein 1H 2D NMR spectra by Interactive Graphics, J. Magn. Reson. (1989) v 24, pp 627-633.
P.J. Kraulis, P.J. Domaille, S.L. Campbell-Burk, T. van Aken & E.D. Laue, Solution Structure and Dynamics of Ras p21.GDP Determined by Heteronuclear Three- and Four-Dimensional NMR Spectroscopy, Biochemistry (1994) v 33, pp 3515-3531.

Other references

S.L. Campbell-Burk, P.J. Domaille, M.A. Starovasnik, W. Boucher & E.D. Laue, Sequential Assignment of the Backbone Nuclei (1H, 15N and 13C) of c-H-ras p21 (1-166).GDP using a novel 4D NMR Strategy, J. Biomol. NMR (1992), v 2, pp 639-646.
A.M. Gronenborn, D.R. Filpula, N.Z. Essig, A. Achari. M. Whitlow, P.T. Wingfield & G.M. Clore, A Novel, Highly Stable Fold of the Immunoglobulin Binding Domain of Streptococcal Protein G, Science (1991), v 253, pp 657-661.
L.-Y. Lian, J.C. Yang, J.P. Derrick, M.J. Sutcliffe, G.C.K. Roberts, J.P. Murphy, C.R. Goward & T. Atkinson, Sequential 1H NMR Assignments and Secondary Structure of an IgG-Binding domain from Protein G, Biochemistry (1991), v 30, pp 5335-5340.
L.-Y. Lian, J.P. Derrick, M.J. Sutcliffe, J.C. Yang & G.C.K. Roberts, Determination of the Solution Structures of Domains II and III of Protein G from Streptococcus by 1H Nuclear Magnetic Resonance, J. Mol. Biol. (1992), v 228, pp 1219-1234.
P.J. Kraulis, Similarity of Protein G and Ubiquitin. Science (1991), v 254, p 581.

Address

e-mail:	ansig@bioc.cam.ac.uk
or	krpx@sgikrpe.sto.pharmacia.se

postal address:
	Per Kraulis
	Pharmacia & Upjohn Inc.
	Biopharmaceuticals R&D, N62:5
	S-112 87 Stockholm
	Sweden

phone:	+46 (8) 695 78 34
FAX:	+46 (8) 695 40 82

Acknowledgments

The following have in different ways helped me to write ANSIG:

T Alwyn Jones, Ernest D Laue, Erling Wikman, Paul C Driscoll, Michael Nilges, Andrew R C Raine, Wayne Boucher, William Broadhurst, Gary R Swanson, Jennifer C J Barna, Peter J Domaille, Hazel Weir, Colin Hardman, Karen M Gould, Jean Thomas, G Marius Clore, Angela M Gronenborn, Johan Kördel, Herbert Baumann, Torleif Härd, The Swedish Natural Science Research Council, Nordisk Industrifond, Siouxsie and the Banshees, The Cure, Philip Glass, Gustav Mahler, Pet Shop Boys, Curve, Jimmy Somerville, Laurie Anderson, Pop Will Eat Itself, Utah Saints, Underworld, Doris Lessing, Ursula K LeGuin, Karl R Popper.

Per Kraulis 10 Apr 1996.